Wheat Researchers Find Ug99 Stem Rust Resistance Gene

Team at K-State, University of California-Davis identifies gene that resists deadly strain of stem rust.

Published on: Jun 27, 2013

Researchers at Kansas State University and the University of California-Davis have identified a gene that provides resistance to one of the worst of enemies of wheat – a deadly strain of stem rust discovered in Uganda in 1999 and named Ug99.

The discovery made by Eduard Akhunov, associate professor of plant pathology at K-State, and his colleague, Jorge Dubcovsky Cal-Davis, may help scientists develop new wheat varieties and strategies that protect the world's food crops from the stem rust pathogen that is spreading from Africa into Asia. Ug99 can cause significant crop losses.

Other Kansas State University researchers on the project included Harold Trick, professor of plant pathology; Andres Salcedo, doctoral candidate in genetics from Mexico; and Cyrille Saintenac, a postdoctoral research associate currently working at the Institut National de la Recherche Agronomique in France. The project was funded by the U.S. Department of Agriculture and Borlaug Global Rust Initiative.

"This gene, Sr35, functions as a key component of plants immune system," said Eduard Akhunov, associate professor of plant pathology at K-State, one of the researchers who discovered the gene. "It recognizes the invading pathogen and triggers a response in the plant to fight the disease."
"This gene, Sr35, functions as a key component of plants' immune system," said Eduard Akhunov, associate professor of plant pathology at K-State, one of the researchers who discovered the gene. "It recognizes the invading pathogen and triggers a response in the plant to fight the disease."

The team has published a paper on their study titled "Identification of Wheat Gene Sr35 that Confers Resistance to Ug99 Stem Rust Race Group," that appears in the current edition of the journal Science.

It identifies the stem rust resistance gene named Sr35, and appears alongside a study from an Australian group that identifies another effective resistance gene called Sr33.

"This gene, Sr35, functions as a key component of plants' immune system," Akhunov said. "It recognizes the invading pathogen and triggers a response in the plant to fight the disease."

Wheat stem rust is caused by a fungus. According to Akhunov, since the 1950s wheat breeders have been able to develop wheat varieties that are largely resistant to stem rust; however emergence of strain Ug99 devastated crops in Uganda and has spread to Kenya, Ethiopia, Sudan and Yemen. It has not reached the U.S.

"Until that point, wheat breeders had two or three genes that were so efficient against stem rust for decades that this disease wasn't the biggest concern," Akhunov said. "However, the discovery of Ug99 showed that changes in the virulence of existing pathogen races can become a huge problem."

As a first line of defense, wheat breeders and researchers began looking for resistance genes among those that had already been discovered in the existing germplasm repositories, he said.

"The Sr35 gene was one of those genes that was discovered in einkorn wheat grown in Turkey," Akhunov said. "Until now, however, we did not know what kind of gene confers resistance to Ug99 in this wheat accession."

To identify the resistance gene Sr35, the team turned to einkorn wheat that is known to be resistant to the Ug99 fungal strain. Einkorn wheat has limited economic value and is cultivated in small areas of the Mediterranean region. It has been replaced by higher yielding pasta and bread wheat varieties.

Researchers spent nearly four years trying to identify the location of the Sr35 gene in the wheat genome, which contains nearly two times more genetic information than the human genome.

Now that the resistance gene has been found, Akhunov and colleagues are looking at what proteins are transferred by the fungus into the wheat plants and recognized by the protein encoded by the Sr35 gene. This will help researchers to better understand the molecular mechanisms behind infection and develop new approaches for control.

Source: Kansas State University News Service